England will be aiming to do one better than the last European Championship, when they reached the final and lost on penalties.
Gareth Southgate has trimmed his 33-man training squad ahead of Friday midnight's deadline and will now step up final preparations for Euro 2024 with a friendly against Iceland.
James Maddison and Curtis Jones were the first to hear the bad news, while Jack Grealish, Harry Maguire, Jarrell Quansah, James Trafford and Jarrad Branthwaite were also missing.
This is our opinion on the final selection:
Goalkeepers
Defenders
Luke Shaw (Manchester United)
Midfielders
Come on
Tags Aaron Ramsdale, Adam Wharton, Anthony Gordon, Arsenal, Aston Villa, Bayern Munich, Belgium, Brazil, Brighton, Brighton and Hove Albion, Bukayo Saka, Caps, Chelsea, Cole Palmer, Conor Gallagher, Crystal Palace, Curtis Jones, Dean Henderson, Declan Rice, Eberechi Eze, England, Everton, Ezri Konsa, Gareth Southgate, Germany, Harry Maguire, Interest, Ivan Toon, Jack Grealish, James Maddison, James Trafford, Jarrad Branthwaite, Jarrod Bowen, Joe Gomes, Johannes Stones, Jordan Pickford, Kieran Trippier, Kobbie Mainoo, Kyle Walker, Lewis Dunk, Luke Shaw, Manchester City, Manchester United, Marc Guéhi, Newcastle United, Ollie Watkins, Phil Foden, Premier League, Real Madrid, Sam Johnstone, Trent Alexander-Arnold, West Ham United